Overview about the localization of nanoparticles in tissue and cellular context by different imaging techniques

• Anja Ostrowski,
• Daniel Nordmeyer,
• Alexander Boreham,
• Cornelia Holzhausen,
• Lars Mundhenk,
• Christina Graf,
• Martina C. Meinke,
• Annika Vogt,
• Sabrina Hadam,
• Jürgen Lademann,
• Eckart Rühl,
• Ulrike Alexiev and
• Achim D. Gruber

Beilstein J. Nanotechnol. 2015, 6, 263–280, doi:10.3762/bjnano.6.25

• these techniques should therefore respect their specific merits and limitations as no single approach combines all desired properties. Keywords: fluorescence lifetime imaging; fluorescence microscopy; histopathology; light microscopic autoradiography; structured illumination microscopy; Introduction

• optical resolution limited to around one millimeter and fail to provide information on the cell or tissue levels [61]. In contrast, the optical resolution of light microscopic autoradiography (LMA) using photoemulsion-covered histological slides is limited by the optical resolving power of the light

• were clearly associated with the red pulp but not within lymphoid follicles (spared dots). (c) Light microscopic autoradiography with numerous radioactive decay-induced signals over Kupffer cells (arrows) in the liver of a mouse (left panel). Signals were sparse in adjacent hepatocytes with larger